Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: Main GUI of the FluoAnalysis toolbox. A multi-tiff file, imported into the GUI with the identified ROIs marked by yellow contours ( top left ). Average projection of the 546 imported frame is visible at the ( top right ), followed by a thresholded binary image and the ROIs in pseudo colors below. ROI detection and ΔF/F 0 calculation parameters are located in the middle column. The calculated ΔF/F 0 traces for all identified ROIs during a control period and application of 1 mM ATP to a hippocampal brain slice expressing GCaMP2 calcium-sensitive fluorescent protein in neurons and astrocytes are visible in the bottom left.
Article Snippet: In conclusion, the FluoAnalysis MATLAB toolbox provides a robust and versatile platform for the integrated analysis of calcium imaging and electrophysiological data, supporting diverse neuroscience research applications.
Techniques: Control, Slice Preparation, Expressing